HAV916V Multidisciplinary Lab Project 1

Responsable

Caroline Desmetz

Crédits : 3 ECTS

Description 

The multidisciplinary lab project, also called “Gene to Protein project”, is a « learning by doing » project. The students will be in charge of the bioproduction of a protein usingE. colias a host. If they follow both parts of the project (1 & 2, like students from Biohealth master), they will start with strain construction and go up to pilot scale production and to purification of the protein.

Bioprocess engineering is a highly interdisciplinary field of study. The students (and future workers in the field), will benefit from project-based learning with an important practical part, where they can actively experience the interconnection between biology, engineering and physical sciences.

The part 1 of the project is a practical course dedicated to the “Strain construction & lab scale production” of a recombinant tagged protein.

Objectives 

This project is for students a unique opportunity to be challenged with a real-life bioprocess-engineering application from gene to protein, with the objective to be immediately  effective for their future employment in biotechnology companies.

This project will provide students additional training in biological concepts, but will also prepare them to communicate and interact with co-workers trained in different disciplines, as many companies operate with multidisciplinary teams working in a basic functional unit. Students will work in multidisciplinary teams since they have been trained in different disciplines (biology, biochemical engineering, pharmaceutical sciences…) but also have acquired different skills through their internships.

The part 1 of the project includes 6 learning objectives (LO):

• LO1: Students will be able to conceive and plan experimental schemes with the equipment made available to them;
• LO2: Students will be able to use and apprehend biology databases (ieNCBI Gene, Nucleotide, Protein) in order to conceive their experiment;
• LO3: Students will be able to adapt established protocols in order to fulfill the project objectives;
• LO4: Students will be able to analyze and interpret the results of experiments with the aim of planning what’s next;
• LO5: Students will be able to document and analyze the data in a correct and scientific way
• LO6: Students are able to work efficiently in a team, and to manage their project.

Know-how and skills in short:

• Cloning design (design and implementation of a cloning protocol, protocol development)
• Protein production engineering (development of culture conditions and purification)
• Project management, communication, and team working in a transversal and interdisciplinary field
• Reporting (laboratory-scale production report)
• …

TP : 27 h

Pré-requis nécessaires  

-Implement an experimental protocol in the field of biology

-Advanced knowledge in molecular biology, cell biology, biochemistry, protein engineering

-UE “ingénierie de la production des protéines recombinantes” (HAV911V).

Pré-requis recommandés 

-Project management

Modalités de contrôles des connaissances

The knowledge assessment is done in full continuous assessment (contrôle continu intégral CCI). Three main learning objectives are rated: carrying out experiments and results, drafting of the production report, project management and team work.